貨號(hào)
產(chǎn)品規(guī)格
售價(jià)
備注
BN40367R-100ul
100ul
¥2360.00
交叉反應(yīng):Mouse(predicted:Human,Rat,Dog,Pig,Cow,Horse,Sheep) 推薦應(yīng)用:WB,IHC-P,IHC-F,ICC,IF,ELISA
BN40367R-200ul
200ul
¥3490.00
交叉反應(yīng):Mouse(predicted:Human,Rat,Dog,Pig,Cow,Horse,Sheep) 推薦應(yīng)用:WB,IHC-P,IHC-F,ICC,IF,ELISA
產(chǎn)品描述
英文名稱 | eIF2C2 |
中文名稱 | 真核翻譯起始因子2C2抗體 |
別 名 | Ago 2; AGO2_HUMAN; Argonaute 2; Argonaute2; dAgo2; eIF 2C 2; eIF-2C 2; eIF2C 2; Eif2c2; Eukaryotic translation initiation factor 2C 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10; Slicer protein. |
研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號(hào) 表觀遺傳學(xué) |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Sheep, ) |
產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù)) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 97kDa |
細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human eIF2C2/Ago2:431-530/859 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產(chǎn)品介紹 | Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5). Function: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. Subunit: Interacts with DICER1 through its Piwi domain and with TARBP2 during assembly of the RNA-induced silencing complex (RISC). Together, DICER1, EIF2C2/AGO2 and TARBP2 constitute the trimeric RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC). Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Note however that the term RISC has also been used to describe the trimeric RLC/miRLC. The formation of RISC complexes containing siRNAs rather than miRNAs appears to occur independently of DICER1. Interacts with EIF2C1/AGO1. Also interacts with DDB1, DDX5, DDX6, DDX20, DHX30, DHX36, DDX47, DHX9, EIF6, ELAVL, FXR1, GEMIN4, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, P4HA1, P4HB, RBM4, SART3, TNRC6A, TNRC6B, UPF1 and YBX1. Interacts with the P-body components DCP1A and XRN1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with RBM4; the interaction is modulated under stress-induced conditions, occurs under both cell proliferation and differentiation conditions and in a RNA-and phosphorylation-independent manner. Interacts with LIMD1, WTIP and AJUBA. Interacts with TRIM71. Subcellular Location: Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8. Post-translational modifications: Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity. Similarity: Belongs to the argonaute family. Ago subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain. SWISS: Q9UKV8 Gene ID: 27161 Database links: Entrez Gene: 27161 Human Entrez Gene: 239528 Mouse Omim: 606229 Human SwissProt: Q9UKV8 Human SwissProt: Q8CJG0 Mouse Unigene: 449415 Human Unigene: 475937 Mouse Unigene: 35512 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 久久嫩草av一级无码专区 | 中文字幕久久一二三区媚药他人妻 | 在线中文在线中文字幕 | 国产又大又黄又长又粗又硬 | 亚洲中文字幕在线视频 | 日本少妇AA一级特黄大片 | 成人做爰www网站视频 | 天天射天天搞天天干绿帽淫妻 | 四川丰满少妇高潮A片 | 妖精视频在线观看国产一区无码 | 五级黄18以上在线观看红桃视频 | 77777少妇AAAAA片毛片 | 白洁老师国产麻豆片 | 亚洲国产精品二二三三区 | 中文字幕不卡少妇 无 | 久久网正在播中文字幕 | 成人网站大胸免费看 | 凹凸人人妻人人做人人玩 | 午夜无码精品一区二区三区99午 | 国产精品三级片在线观看 | 成人人妻A片一区二区 | 少妇做爰免费8片免费观看 做爰高潮A片〈毛片〉 | 无遮无挡试看120秒高潮 | 成年免费视频黄网站在线观看 | 五月婷婷乱伦海角出品 | 无码秘 人妻一区二区三区 国产精品一区二区三区在线 | 国产又粗又猛又爽又黄的 | 国产色情一级二级三级视频 | 人人妻人人玩人人澡人人爽 | 欧美性夜黄A片爽爽免费视频 | 91精品久久久久久久99蜜桃 | 国产资源在线播放 | 91丨老师丨国产丨地址 | 成人国产片女人爽到高潮 | 日本人妻伦在线中文字幕 | 日本午夜电影 在线 | 国产小骚货性爱在线观看 | 啪啪啪欧美吸奶三级视频 | 国产丰满熟妇蜜臀AV高潮 | 亚洲熟伦熟女新五十路熟妇 |